1D 1H-NMR

¹H-NMR is the application of NMR with respect to proton nuclei. In order to record the molecules of interest only, deuterated solvents are used, e.g., D₂O instead of H₂O. D₂O can also be used as a lock signal.

In 1D spectra, the chemical shifts are depicted on the X-axis and the intensity of the signal on the Y-axis. The intensity depends on the number of nuclei giving rise to the respective signal. 1D spectra are useful for small molecules/proteins only, because the interpretation of many overlapping signals is extremely complex.

Figure 1: 1D ¹H-NMR of alanine