1H-NMR is the application of NMR with respect to proton nuclei. In order to record the molecules of interest only, deuterated solvents are used, e.g., D2O instead of H2O. D2O can also be used as a lock signal.

In 1D spectra, the chemical shifts are depicted on the X-axis and the intensity of the signal on the Y-axis. The intensity depends on the number of nuclei giving rise to the respective signal. 1D spectra are useful for small molecules/proteins only, because the interpretation of many overlapping signals is extremely complex.

Figure 1: 1D 1H-NMR of alanine