Enzyme Kinetics

Introduction

• ADH enzyme kinetics

Lab Theory

• Enzyme
  • ADH
  • Active site
  • Substrate
  • Co-factor
  • Co-factor for alcohol dehydrogenase
  • Product
• Enzyme kinetic assay
• Michaelis-Menten
  • Michealis-Menten equation
  • Reaction rate
  • Initial reaction rate
  • K_m
  • V_max
  • k_cat
• Inhibitors
  • Competitive inhibition
  • Un-competitive inhibition
  • Mixed / non-competitive inhibition
• Master mix
• Spectrophotometer