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Welcome to the Enzyme Kinetics Lab
LabsterX.ENK.2014
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Introduction
ADH enzyme kinetics
Lab Theory
Enzyme
ADH
Active site
Substrate
Co-factor
Product
Enzyme kinetic assay
Michaelis-Menten
Reaction rate
K
m
V
max
k
cat
Inhibitors
Competitive inhibition
Uncompetitive inhibition
Mixed/noncompetitive inhibition
Master mix
Spectrophotometer