Array-CGH Experiment

The array CGH steps are described as follows:

Figure 1:The process of Array CGH

• The DNA is treated with enzymes to cut it into smaller pieces

• The sample and reference DNA are labeled with different colored fluorescent probes. The reference DNA is labeled with Cy5, a red fluorescent dye, while the sample DNA is labeled with Cy3, a green fluorescent dye.

• Both samples are mixed and co-hybridized to the array. The array contains several hundred thousand probes (e.g. oligonucleotides) representing sequences from all chromosomes.

• Analysis