Automated injection

Figure 1. This is an automated sampler, where a robot will inject the samples into the HPLC instrument.

In contrast to the manual injection, a robot is used to inject the sample into the instrument. The efficiency is much improved as compared to the manual injection because you can inject many more sample in a short time. Because of this the automated injected is used more in the lab.

Figure 2. This is the vials used for the automated sampler. Samples are stored in these vials and they are placed in a specific order in the auto sampler.

It consists of an auto sampler (see Figure 1) where you place all your samples in different numbered positions. They can be placed in vials (see Figure 2) or plates with several wells. The only thing that rests is programming with a software the sequence of injections. A robotic needle will move to the chosen position, take the desired volume and transfer it into a loop from where it is injected into the mobile phase.