E.coli is one of the most widely used and well-studied expression systems. This system is excellent for expression of functional non-glycosylated proteins, since the production of glycosylated proteins require a Golgi apparatus, which E. coli cells do not possess. Bacteria did not develop a sophisticated mechanism for performing posttranslational modifications.
Advantages of E.coli as an expression system.
Disadvantages of E.coli as an expression system.
Figure 1. Bacteria expression system.