Benedict's solution

Benedict's solution is used to detect reducing sugars, typically monosaccharides or disacharides. It will show a positive result for reducing sugars such as glucose, fructose, lactose, maltose, galactose. It will show a negative result for non-reducing sugars such as sucrose, starch.

There are two test tubes. The first test tube is the negative result and contains a blue solution. The second test tube is the positive result and contains a red-brown solution.

Figure 1. (-) is a negative result; (+) is a positive result.

Benedict's solution is a blue colored liquid that contains copper sulfate. Copper binds to oxygen in the free aldehyde or ketone group forming a copper oxide. The copper oxide transmits a brown color.

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Food biochemistry test

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Food biochemistry assay