Cell viability calculation

To calculate cell viability of the cell culture, Trypan blue is normally used. This is a vital stain that is permeable to cells depending on their membrane integrity. Therefore, cells visualized in blue under the microscope will be assumed as dead while the rest would be living cells. To use Trypan blue you just need to add it to the cell solution (commonly using 1:2 ratio), count the total number of living cells as well as the total number of dead cells, and calculate the percentage.

Example:

Live cell count = 2.86 x 106 cells/mL two point eighty six times ten to the sixth cells per milliliter

Dead cell count = 1.34 x 105 cells/mL one point thirty four times ten to the fifth cells per milliliter

Total number of cells = 2.994 x 106 cells/mL two point nine hundred ninety four times ten to the sixth cells per milliliter

(2.86 x 106 / 2.994 x 106) x 100 = 95.5% two point eighty six times ten to the sixth divided by two point nine hundred ninety four times ten to the sixth times a hundred equals to ninety five point five percent viability