ChIP-exo protocol overview

The ChIP-exo protocol includes several steps, but these are the main parts of the process:

• Cell culture and Cross-linking.

• Fragmentation and preparation of antibody-TF complex.

• Antibody-TF complex binding to magnetic beads.

• On-bead enzymatic modifications.

• Elution from magnetic beads.

• Off-bead enzymatic modifications.

• PCR amplification.

• Library purification.

• TOPO cloning and Sanger sequencing.