Chromosome Fixation

The fixation reagent will fix the chromosome so it will keep its size and structure during the preparation. It also arrests the process of hypotonic swelling and all metabolic processes of the cells. The fixed cells in suspension can be kept for years in a freezer. The fixation reagent (also called Carnoy's solution) is composed of three parts of absolute methanol and one part of glacial acetic acid.

Referred from: