Dpn I purification

Dpn I is a restriction enzyme that specifically cleaves DNA containing methylated adenine (mA) in the recognition sequence GmATC. It is often used after PCR to remove the remaining template DNA, especially in molecular cloning and site-directed mutagenesis experiments, when the template DNA is a plasmid. Since the PCR-amplified DNA is not methylated, DpnI will not cleave it and will only cleave the template DNA.