E-cadherin

E-cadherin is normally located in the cell membrane. When performing an immunostaining experiment with fluorescence-labeled antibodies, it can be seen at the cell borders. However, when undergoing an EMT event, its localization changes and the protein moves to the cytosol. When using fluorescence-labeled antibodies in an immunostaining procedure, this is observed as the protein is distributed all over the cell.

F-actin F actin

F-actin F actin is the most abundant component of the cytoskeleton and is therefore located inside the cell. When undergoing EMT, its localization does not change, but there is a reorganization of the filaments. In epithelial cells, they change from thin cortical bundles into thick, parallel, contractile bundles, also known as stress fibers, which is proper of a mesenchymal phenotype.