ELISA high background
High background refers to excessive color development or high optical density readings.
| Possible cause | Troubleshooting |
|---|---|
| Poor washing | Check the water quality. Use the highest number of washes recommended |
| Laboratory temperature is too high or too low | Mantain the room temperature within 18-25 oC |
| Reagents were intermixed, contaminated or prepared incorrectly | Ensure correct reagents are used and not expired |
| Substrate has deteriorated. | Make sure the substrate is colorless |