ELISA low OD reading
The optical density (OD) measurement results in low or poor signals that show as insufficient color development in 96-well plate.
| Possible cause | Troubleshooting |
|---|---|
| Too many wash cycles were used | Try using the lowest number of washes recommended |
| Incubation periods were too short | Follow incubation time protocol |
| Not enough antibody | Increase the concentration of antibody |
| Reagents were expired or intermixed | Verify the expiration dates of the reagents. |