ELISA no signal
No signal means no color development in the ELISA assay.
| Possible cause | Troubleshooting |
|---|---|
| Reagents were used in the wrong order or expired | Check the reagent package |
| Incorrect, expired or no detection antibody is added | Add the appropriate detection antibody |
| Samples are not added to the diluent | Check that samples were added to the diluent |
| Substrate solution is not added | Add substrate solution |