ELISA poor standard
Standard is required to determine unknown concentrations. Poor standard has R2 below 0.99.
| Possible cause | Troubleshooting |
|---|---|
| Standard solution is not added. | Add the standard solution. |
| Blank has higher OD than the lowest standard. | Minimize the edge effect by covering the well plate with microplate seal. Avoid working near heat sources. |
| Mistake in serial dilution. | Pay attention to volumes of standard and buffer solutions |
| Standard preparation might have denature. | Check the expiration dates. |