Hematocrit

The volume of red blood cells is also defined as hematocrit. After centrifugation of a blood tube, two layers can be seen: the upper phase consists of the plasma, while the red blood cells sink to the bottom of the tube, which is visible as a red layer (Figure 1). White blood cells and platelets settle in the interface between both layers. A low hematocrit value can be an indicator for anemia.

This image shows a diagram with two purple-capped test tubes. The first test tube, on the left, is filled with whole blood before processing. An arrow labeled Centrifugation points from the first tube to the second tube on the right. The second tube shows the blood sample separated into three distinct layers, which from top to bottom are plasma in yellow, White Blood Cells and Platelets in white, and Red Blood Cells in red. This red blood cells at the bottom of the tube represent Hematocrit, which is the percentage by volume of red cells in the blood.

Figure 1: Schematic representation of the determination of the hematocrit.