Immunofluorescence protocols

Immunofluorescence protocols are characterized by the use of fluorophores linked to antibodies to detect specific antigens in cells or tissues.

The immunofluorescence can be detected by different types of equipment, such as fluorescence microscopes, ideal for exploring qualitative changes in the samples, or flow cytometry apparatus like FACS, used when a more quantitative result is needed.

Some considerations are needed when using fluorescence microscopy: