Liquid chromatography
Liquid chromatography is a type of chromatography where the mobile phase is a liquid, while the stationary phase can be solid or liquid.
According to the interaction between phases, which will define the method of sample separation, there are several types of liquid chromatography:
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Normal phase chromatography.
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Reverse phase chromatography.
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Affinity chromatography.
Figure 1. General path flow in liquid chromatography.
Liquid chromatography typically uses the gravity force to pass the mobile phase through the stationary phase. However, when a significantly higher operational pressure (50-350 bar) is used to move the mobile phase through the system, the technique is called High-Performance liquid chromatography (HPLC).