Mallory staining method

The Mallory staining method makes use of three different dyes. This method has the advantage of visualizing a larger number of tissue structures. Mallory stain combines aniline blue, which stains connective tissue, extracellular matrix, glycoproteins, and mucus, orange G, which stains proteins, and the dark red fuchsin, which stains RNA and DNA.

Tissue from the crypt of Lieberkühn that has been stained with the Mallory staining method. Lining the crypt are cells that contain DNA and are darkly stained by fuchsin dye. Acting as a boundary to separate two rows of cells, there is a line of connective tissue stained with aniline blue dye. Within the crypt are lightly dyed circles and an unstained area, which is the lumen of the crypt.

Figure 1: Crypt of Lieberkühn stained with the three Mallory dyes


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