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Microliter pipette instructions

After selecting a pipette with an appropriate volume, you are ready to transfer liquids using the following steps.

Drawing up liquid

Before drawing up liquid, make sure you add a sterile tip to the pipette. Then press the plunger from the rest position to the first stop.

On the left, the rest position, first stop, and second stop of the pipette plunger. The release position is the neutral plunger position farthest away from the body of the pipette. Pressing on the plunger part way will move it to the first stop, which is closer to the pipette body but not yet touching it. The second stop is reached when the plunger is pressed all the way to the body of the pipette. Next, the positioning of the pipette for picking up and releasing liquids. For drawing up a liquid, the pipette is shown perpendicular to the surface of the liquid being picked up. For releasing a liquid, the pipette is shown at a 45 degree angle to one side of the receiving container.

Hold the pipette vertically and dip the pipette tip 3-4 mm into the liquid and carefully release the plunger in a controlled manner (1).

Dispensing liquid

To dispense liquid hold the pipette tip against the side of the tube at a 30-40 deg angle or dip the tip beneath the surface of the liquid already contained in the tube (2).

Then press the plunger to the second stop to make sure no fluid remains inside the tip. Discard the tip into the trash after each pipetting event by using the ejector.

Referred from:

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Microliter pipette