Molecular Cloning Introduction
Welcome to Labster's Molecular Cloning Lab
Here, you will learn about the molecular cloning techniques including how to prepare the DNA insert and vector, and how to perform restriction enzyme digestion and ligation followed by transformation, plating, and antibiotic selection. Finally you will perform a DNA damage experiment using UV radiation and find out whether RAD52 is required for DNA repair.
You will be able to find all the related theory in this Wiki page needed for completing the case.
Good luck!
Molecular Cloning Lab Progression
- Introduction
- DNA isolation
- Restriction enzyme digestion and ligation
- Vector assembly
- Transformation and selection
- DNA damage experiment
- Conclusion