Welcome to the Protein Synthesis Simulation! In this simulation, you will learn how to synthesize a recombinant protein called recombinant human erythropoietin (rhEPO), which will be used for anemia therapy. However, rhEPO is also used illegally by athletes for blood doping. You will employ mass spectrometry techniques to find which athletes have illicitly used the rhEPO.
Here we have gathered all the relevant theory material to boost your learning.
Click on the blue button to choose the mass spectrometry data of rhEPO expressed in CHO and E.coli. There are three graphs:
The one on the left shows the total mass of the molecule detected. The peaks in this graph correspond to the molecular mass of that particular protein.
The top right graph shows the overview of the peptide sequence. The peaks in this graph represent one amino acid in the peptide sequence.
The bottom right graph shows the magnification of the peptide sequence shown in the top graph.
The peaks in this graph represent one amino acid in the peptide sequence. Notice that the diagram of glycan marks the site of glycosylation.