Restriction enzyme cloning

Restriction enzyme based molecular cloning is the "classic" cloning method and is still one of the most popular techniques. In principle, the plasmid assembly involves these following steps:

Higher success rates are achieved when using DNA fragments with 5' or 3' sticky/overhang ends rather than assembling DNA fragments with blunt ends.

  • 3A assembly is a method used to efficiently assemble two BioBricks.