# Aerobic dive limit

The measurement of Weddell seals and emperor penguins blood lactate concentration pre- and post-dive led to the definition of aerobic dive limit (ADL) as the dive duration after which there is an increase in the post-dive concentration of lactate.

For all other species, where no pre- and post-dive lactate measurement is made, the aerobic dive limit has been calculated by dividing usable oxygen stores by an estimation of the rate of oxygen consumption during diving.

In this simulation, we will use both approaches, as Weddell seals can, in fact, dive for at least 2-3 times longer than their ADL. This means that at ADL, there is still some usable oxygen remaining in the stores.

Below, you can find the equations used to calculate oxygen stores and aerobic dive limit from stores.

**Calculate oxygen stored in the lungs**

VO_{2} in lungs (mL) = lung volume (L) x O_{2} in lung(mL/L)

**Calculate oxygen stored in the blood**

VO_{2} in blood (mL) = O_{2} in hemoglobin(mL/g) x hemoglobin(g/kg) x blood mass (kg)

**Calculate oxygen stored in the muscle**

VO_{2} in muscle (mL) = O_{2} in myoglobin(mL/g) x myoglobin(g/kg) x blood mass (kg)

Total O_{2} stores can then be calculated by the sum of O_{2} in all compartments. Further dividing this value by the total body mass(kg)provides a relative and more comparable measure (in mL/kg).

Once we know the total oxygen and an estimation of the factorial increase in O_{2} consumption during diving, we can **calculate the O _{2} consumption during diving**:

Diving O_{2} consumption = factorial increase x resting O_{2} consumption (mL/min)

**And then, finally, the aerobic dive limit:**

ADL (min) = total O_{2} in stores (mL) / rate of O_{2} consumption during diving (mL/min)