Separation of pigments

The aim of this experiment is to use paper chromatography to separate the pigments in leaves, such as spinach, and flowers, such as rose and marigold, and then determine the R_f values of the components.

• Paper chromatography theory

• Experimental set up

• Experimental procedure

• How to note your observations

• Experimental precautions

References:

• NCERT Chromatography handbook