Basic steps of ELISA

A wide variety of ELISA types are available for different applications. However, all ELISA types still require the same basic elements:

• Coating/capture: Immobilization of the antibody or antigen to the surface of the microplate wells
• Blocking: Addition of irrelevant protein or other molecules to cover all unsaturated surface-binding sites of the microplate wells
• Probing/detection: Incubation with the labeled antigen or antibody
• Signal measurement: Detection of the signal generated via the labeled antigen or antibody