qPCR experiment

During the qPCR experiment the target DNA is copied repeatedly just like in a normal PCR-reaction. The SYBR-green in the mix binds to the double stranded DNA molecules which makes it fluorescent. In the end of every extension step a photograph is taken and the strength of the fluorescence is quantified.

Contrary to normal PCR these measurements enable you to quantify the amount of DNA fragments in real time.

After the last cycle the qPCR thermocycler measures the fluorescence while steadily increasing the temperature to record the melting curve.

The obtained measurement are sent to a computer for analysis.