USER overhangs
Uracil excision cloning relies on the creation of cohesive 3' overhangs in the DNA fragments to assemble by the specific 5' overhangs designed for the USER primers (Figure 1).
Figure 1. USER overhangs comparison. U represents Uracil.
Uracil excision cloning relies on the creation of cohesive 3' overhangs in the DNA fragments to assemble by the specific 5' overhangs designed for the USER primers (Figure 1).
Figure 1. USER overhangs comparison. U represents Uracil.