Cell Culture Basics: Plate, split and freeze human cells

In the Cell Culture Basics Simulation, you will use the aseptic technique to culture mammalian cells. You will perform a cell passage and freeze some cells for a backup stock. You will use microscopy to distinguish different cell morphologies and investigate how cells grow. Will you be able to maintain your cells alive and free from contamination?

• Cell culture

  • Applications of cell culture

  • Cell culture environment

    • Cell culture media

    • Physico-chemical conditions

    • Mammalian cell morphology

• Aseptic technique

  • Sterile work area

  • Sterile handling

  • Biological contamination

• Thawing frozen cells

• Cell growth

  • Doubling time

• Cell passaging

  • Trypsin

  • Cell counting

    • Manual cell counting

    • Cell viability

• Cryopreservation