ChIP-exo

In the ChIP exo simulation, you will perform the chromatin immunoprecipitation with exonuclease treatment (ChIP-exo) protocol to elucidate the acid stress mechanism of Escherichia coli. Will you be able to complete all the steps correctly to understand how E. coli survive in acid stress conditions?

Following is a list of the relevant theory pages:

• Chromatin immunoprecipitation definition

• ChIP-exo definition

• Exonuclease definition

• Cell culture conditions for ChIP-exo

• Cross-linking protein-DNA in ChIP-exo

• Fragmentation

• Preparation of antibody-transcription factor complex

• Magnetic beads

• Washing procedure in ChIP-exo

• Reparation of DNA sticky ends

• DNA dA tailing

• ChIP exo-adapters

• Reverse crosslinking

• Protein removal and DNA purification in ChIP-exo procedure

• PCR enrichment

• TOPO cloning

• Sanger sequencing