Confocal Microscopy

Welcome to Labster’s Confocal Microscopy Simulation!

In this simulation, you will learn about the benefits of using a confocal microscope over other optical imaging techniques. You will have a close look at the setup of this type of microscope and how to operate it. Finally, you will be trained in how to acquire pin sharp micrographs and mind-blowing 3D renderings of your sample. Your overall task will be to use confocal microscopy to investigate a mystery fungus found on a barley plant from your uncle’s crop fields. Are you ready to identify the fungus and give your uncle advice on a potential treatment?

You will be able to find all the related theory needed to complete the course in this theory page.

Good luck and have fun!

• Plant Diseases
  • Powdery Mildew
    • Fungicide
• Fluorophores
• Confocal Microscopy
  • Lasers
  • Pinhole
  • Photo-multiplier tube (PMT)
  • Scanning Mirror
  • Scanline Speed
  • Gain
  • Z-stack

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Acknowledgement The content of these theory pages has been developed based on the resources provided by:

• OpenStax College, Biology. (OpenStax CNX. Mar 13, 2015)

• The image of "Powdery mildew infected barley" was kindly provided by Clemson University, USDA Cooperative Extension Slide Series