ELISA

In this lab you will learn the basic of immunoglobulins and ELISA techniques. You will explore the ELISA methods and their modifications. Following is a list of the relevant theory pages:

• Hemophilia
• Factor IX
• Antibody
• ELISA
  • Steps of ELISA
  • ELISA troubleshooting
• ELISA Data analysis
  • Spectrophotometry
  • Negative control
  • Positive control
  • Standard curve
  • Data fit
• Dilution

Acknowledgement

The content of these theory pages has been developed based on the resources provided by:

• OpenStax College, Biology (OpenStax CNX. 14 Mar 2015).

• Hopfner, K.P., Lang, A., Karcher, A., Sichler, K., Kopetzki, E., Brandstetter, H., Huber, R., Bode, W., Engh, R.A. (1999).Coagulation factor IXa: the relaxed conformation of Tyr99 blocks substrate binding. Structure Fold. Des. 7: 989-996