CRISPR-Cas applied to TGF-beta induced EMT

In this simulation, you will set up an immunofluorescence protocol to understand the molecular hallmarks of the Epithelial-Mesenchymal Transition (EMT) induced by TGF-beta in a breast cancer cell line, and will design and perform a CRISPR-Cas9 experiment to knock-out a gene involved in EMT as a possible therapeutic approach. Remember that you can access the theory section at any moment in your LabPad while playing the simulation.

Below is a list of all the relevant theory pages to boost your learning:

• Epithelial-Mesenchymal transition
• TGF-beta signaling
• Immunofluorescence protocols
  • Preparation of cells
  • Use of antibodies
  • Use of fluorescent probes
• CRISPR-Cas
  • CRISPR-Cas components
  • CRISPR-Cas9 molecular mechanism
  • Generating knock-out cells with CRISPR-Cas9

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• E-cadherin
• F-actin
• Experimental controls