CRISPR-Cas applied to TGF-beta induced EMT
In this simulation, you will set up an immunofluorescence protocol to understand the molecular hallmarks of the Epithelial-Mesenchymal Transition (EMT) induced by TGF-beta in a breast cancer cell line, and will design and perform a CRISPR-Cas9 experiment to knock-out a gene involved in EMT as a possible therapeutic approach. Remember that you can access the theory section at any moment in your LabPad while playing the simulation.
Below is a list of all the relevant theory pages to boost your learning:
- Epithelial-Mesenchymal transition
- TGF-beta signaling
- Immunofluorescence protocols