Microscopy

In this lab you will learn different microscopy techniques. You will examine samples from a chicken intestine, which is infected with a virus that is able to suppress the immune system of the chicken. You will start with a stained light microscopy slide and identify different structures of the small intestine. To see even more detail, you will use a transmission electrone microscope to examine cellular structures in high detail. You will end the mission upon localizing the virus in the sample by using fluorescence microscopy.

Following is a list of the relevant theory pages:

• Microscopy

  • Light Microscopy

    • Light microscope

    • Specimen preparation

    • Staining

  • Fluorescent microscopy

    • Fluorescent dyes

    • Fluorescent microscope

  • Electron microscopy

    • TEM

• Small intestine

  • Intestinal epithelium

  • Inflammation of the intestine

  • Coeliac disease

  • Malabsorption syndrome in chicken