Next Generation Sequencing

Introduction

• Next Generation Sequencing case: The ancient Greenlandic man

Lab Theory

• DNA
  • Ancient DNA sample
• Next Generation Sequencing
  • Fragmentation
  • End-repair
  • A-overhang
  • Adapter ligation
  • PCR amplification
• Cluster generation
• Sequencing process
• NGS data analysis
• Ancient Greenland SNP