In the Mammalian Transient Protein Simulation, you will use the ExpiCHO expression system to perform a transient protein production in ExpiCHO-S cells. You will grow a suspension culture of ExpiCHO-S cells, prepare both the reagents and the cells for transfection, and learn which protocol you should use, depending on the desired protein yield. Will you be able to keep your cells healthy to maximize protein expression?

Following is a list of the relevant theory pages: