RNA Extraction: Sample and purify mRNA from pigs

In this simulation, you will learn the basics of RNA extraction from live tissue to obtain a purified mRNA sample. This preparation has many uses, for example if you want to sequence your genes and analyze their expression profiles.

Unlike DNA, RNA is a single-strand and very fragile molecule which can quickly deteriorate without proper care in your experiments. This is why good training and vigilance are very valuable.

The experiments in this simulation will have you purify samples from live pigs to work on an obesity project.

You can find the relevant topics approached in this simulation in the following sections:

• Obesity and genes

• Gene expression

• RNA isolation

• mRNA purification