Tissue Engineering

In this Tissue Engineering Lab, you will learn about principles of tissue engineering in articular cartilage regenerative medicine. You will explore types of polymers and hydrogels to synthesize hydrogels.

• Articular cartilage
• Tissue engineering
  • Hydrogels
  • Scaffold
• Polymer
• Crosslinking
  • Ionic crosslinking
  • Radical crosslinking
  • Michael-addition crosslinking
  • Enzymatic crosslinking
• Mechanical properties
  • Rheology
  • Gelation

Reference

• Chen, T.(2003). Enzyme-catalyzed gel formation of gelatin and chitosan: potential for in situ applications. Biomaterials, 24(17), 2831-2841.
• Choi, D.(2008). Preparation of poly(ethylene glycol) hydrogels with different network structures for the application of enzyme immobilization. Bio-Medical Materials and Engineering, 18, 345-356.
• Ehrbar, M.(2007). Enzymatic formation of modular cell-instructive fibrin analogs for tissue engineering. Biomaterials, 28, 3856-3866.
• Higham, A. K. (2014). Photo-activated ionic gelation of alginate hydrogel: real-time rheological monitoring of the two-step crosslinking mechanism. Soft Matter, 10(27), 4990-5002.
• Jeon, O.(2007). Mechanical properties and degradation behaviors of hyaluronic acid hydrogels cross-linked at various cross-linking densities. Carbohydrate Polymers, 70(3), 1, 251-257.
• Jin, R.(2010). Synthesis and characterization of hyaluronic acid–poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair. Acta Biomaterialia, 6(6), 1968-1977.
• Kuo, C. K. (2001). Ionically crosslinked alginate hydrogels as sca!olds for tissue engineering: Part 1. Structure, gelation rate and mechanical properties. Biomaterial, 22, 511-521.
• Lutolf, M. P., & Hubbel, J. A. (2003). Synthesis and Physicochemical Characterization of End-Linked Poly(ethylene glycol)-co-peptide Hydrogels Formed by Michael-Type Addition. Biomacromolecules, 4, 713-722.
• Park, Y. D. (2003). Photopolymerized hyaluronic acid-based hydrogels and interpenetrating networks. Biomaterial, 24, 893-900.
• Tibbit, M. W. (2013). Mechanical Properties and Degradation of Chain and Step-Polymerized Photodegradable Hydrogels. Macromolecules, 46, 2785-2792.
• Vorvolakos, K.(2011). Ionically cross-linked hyaluronic acid: wetting, lubrication, and viscoelasticity of a modified adhesion barrier gel. Medical Devices, 41-10.