In this Tissue Engineering Lab, you will learn about principles of tissue engineering in articular cartilage regenerative medicine. You will explore types of polymers and hydrogels to synthesize hydrogels.

• Articular cartilage
• Tissue engineering
  • Hydrogels
  • Scaffold
• Polymer
• Crosslinking
  • Ionic crosslinking
  • Radical crosslinking
  • Michael-addition crosslinking
  • Enzymatic crosslinking
• Mechanical properties
  • Rheology
  • Gelation

Reference

• Chen, T.(2003). Enzyme-catalyzed gel formation of gelatin and chitosan: potential for in situ applications. Biomaterials, 24(17), 2831-2841.
• Choi, D.(2008). Preparation of poly(ethylene glycol) hydrogels with different network structures for the application of enzyme immobilization. Bio-Medical Materials and Engineering, 18, 345-356.
• Ehrbar, M.(2007). Enzymatic formation of modular cell-instructive fibrin analogs for tissue engineering. Biomaterials, 28, 3856-3866.
• Higham, A. K. (2014). Photo-activated ionic gelation of alginate hydrogel: real-time rheological monitoring of the two-step crosslinking mechanism. Soft Matter, 10(27), 4990-5002.
• Jeon, O.(2007). Mechanical properties and degradation behaviors of hyaluronic acid hydrogels cross-linked at various cross-linking densities. Carbohydrate Polymers, 70(3), 1, 251-257.
• Jin, R.(2010). Synthesis and characterization of hyaluronic acid–poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair. Acta Biomaterialia, 6(6), 1968-1977.
• Kuo, C. K. (2001). Ionically crosslinked alginate hydrogels as sca!olds for tissue engineering: Part 1. Structure, gelation rate and mechanical properties. Biomaterial, 22, 511-521.
• Lutolf, M. P., & Hubbel, J. A. (2003). Synthesis and Physicochemical Characterization of End-Linked Poly(ethylene glycol)-co-peptide Hydrogels Formed by Michael-Type Addition. Biomacromolecules, 4, 713-722.
• Park, Y. D. (2003). Photopolymerized hyaluronic acid-based hydrogels and interpenetrating networks. Biomaterial, 24, 893-900.
• Tibbit, M. W. (2013). Mechanical Properties and Degradation of Chain and Step-Polymerized Photodegradable Hydrogels. Macromolecules, 46, 2785-2792.
• Vorvolakos, K.(2011). Ionically cross-linked hyaluronic acid: wetting, lubrication, and viscoelasticity of a modified adhesion barrier gel. Medical Devices, 41-10.