Uracil based excision cloning (USER): Cloning made simple

In this simulation, you will insert three genes related to the beta-carotene metabolic pathway into an E. coli strain, using the USER cloning method. Remember that you can access the theory section at any moment in your LabPad while playing the simulation.

Following is a list of all the relevant theory pages to boost your learning:

• USER cloning definition

  • USER primer design

  • USER overhangs

  • USER enzymes

  • Controls in USER cloning

• Touchdown PCR

• Dpn I purification

• USER troubleshooting guide

• Beta-carotene