Western blotting or Western blot is an important method in protein identification. A Western blot can detect one specific protein in a solution that contains numerous other proteins. The term Western blot specifically refers to the transfer of proteins and their detection using antibodies.
Figure 1. Western blot experimental steps. Following sample preparation (A), the samples are loaded onto an SDS polyacrylamide gel (B) and separated by gel electrophoresis. In the next step, the separated proteins are transferred onto a membrane (C) and detected by antibodies targeting the protein of interest (D). Finally, the detected protein bands are visualized on the membrane (E).